Cells were plated (3000 cells per well, 96-well plates) and, after culture for 24 h for cell attachment, they were treated with c-PTIO (60 μM in MCF-7 and 40 μM in
Tamoxifen regulates macrophage activation by inducing PI3K-NRF2 pathway, caspase-1 formation and by modulating lipid metabolism and calcium homeostasis, as well as other possible targets identified in other cell types, such as PKC and oxidative stress
While Popular answers (1) Philip E Lapinski
The purpose of sample incubation was to achieve equilibration of possible Tamoxifen binding with various cell culture proteins, and to avoid too much analyte degradation in the cell culture medium
Higher doses (100 nM) of endoxifen and 4HT, as well as a low dose Razandi M, Pedram A, Jordan VC, Fuqua S, Levin ER: Tamoxifen regulates cell fate through mitochondrial estrogen receptor beta in breast cancer
The effect of 4-hydroxytamoxifen (4OH-TAM), the potent anti-estrogenic metabolite of tamoxifen, on the radiosensitivity of MCF-7 cells irradiated in vitro was determined
10107) at 1 mM; the final concentration was 1 µM (the final concentration of ethanol in the culture dishes was 0
2 µM 4HT (4-hydroxi-tamoxifen) (Sigma-Aldrich) for MEFs 4-Hydroxytamoxifen (4-HT) is the active metabolite of tamoxifen which binds estrogen receptors (ER) and estrogen-related receptors (ERR) with estrogenic and anti-estrogenic effects
rapamycin was examined by MTT analysis after incubation of the cells in the indicated concentrations of the drugs
Here, we demonstrate that endoxifen-mediated recruitment of ERα to known
Furthermore increasing the concentration of tamoxifen did not further decrease cell viability (data not shown) hence a range from 0 to 25 nM was used in subsequent experiments
Microglia are the main TAK1-expressing
Continuous use of tamoxifen exceeding 5 years is associated with an increased risk of endometrial cancer and blood clots
If rapid induction of Cre-ER is required, as in cell lineage studies during embryogenesis, treatment of mice with 4HT in the place of tamoxifen will provide far more precise timing of Cre-ER induction